2019-07-12 08:48:362019-02-08 18:10:57
The protein
Paralogous protein(s)
[[this]]
Biological materials
Mutant
MGNA-B639 (yvcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1638 NBRP B. subtilis, Japan]
GP231 (''amyE::P[[gene|rpsJ]]-lacZ, [[gene|whiA]]::cat'') available in [SW| Jörg Stülke]'s lab; plasmid pGP1468 was used to disrupt the gene.
BKE34750 (Δ[[gene|whiA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
BKK34750 (Δ[[gene|whiA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
MGNA-B639 (yvcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1638 NBRP B. subtilis, Japan]
GP231 (''amyE::P[[gene|rpsJ]]-lacZ, yvcL::cat'') available in [SW| Jrg Stlke]'s lab; plasmid pGP1468 was used to disrupt the gene.
BKE34750 ([[gene|whiA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
BKK34750 ([[gene|whiA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAGCCACCTCATTTCA, downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
Biological materials
Expression vectors
pGP1469 for expression in ''B. subtilis'' (in [SW|pGP382], with C-terminal STREP-TAG), available in [SW| Jörg Stülke]'s lab
pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP1469 for expression in ''B. subtilis'' (in [SW|pGP382], with C-terminal STREP-TAG), available in [SW| Jrg Stlke]'s lab
pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jrg Stlke]'s lab
pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jrg Stlke]'s lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jörg Stülke]'s and [SW|Boris Görke]'s labs
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jrg Stlke]'s and [SW|Boris Grke]'s labs
Biological materials
FLAG-tag construct
GP782 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
GP782 (''spc'', based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab